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Company name: Ladd Porter (Beijing) Biotechnology Co., Ltd.

Contact: Zhang Wei

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Beacon single cell photoconductive system

Beacon single cell photoconductive system

  • Taxonomy:Beacon single cell photoconductive system

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  • Date of release:2020/02/26
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Detailed introduction

The beacon single cell photoconductive system integrates the photoelectric positioning technology and microfluidic technology to realize the high-throughput cell biology research based on single cell in the nano upgrading cell of the chip.

Technological breakthrough

The existing single cell operation technologies in the development of monoclonal cell lines, such as limited dilution method, flow sorter and other technologies, although single cells can be obtained, they often damage cells and interfere with the normal state of cells, Therefore, the proportion of clone formation is very low, and there are some shortcomings, such as poor monoclonal ability, difficult observation, long time, low flux, poor plasticity, etc. In addition, it is not enough to get single cell, and the process of cell culture, detection and export needs appropriate solutions. If we use the non integrated scheme, we often have problems such as difficult culture, difficult analysis, difficult export or poor repeatability, which will wipe out the efforts to obtain single cells in the early stage.

The beacon single cell photoconductive system can directly operate and culture a single target cell from the beginning of the experiment, with reliable results and high efficiency. This system will have a unique optoelectronic positioning technology Combined with novel nanofluidic design, it can not only select single cells accurately on a chip, but also directly culture, use multiple different channels in real time and continuously, detect cell status, conduct multiple experiments on single cells or monoclonal, and derive target cells and target clones according to the specific results read during the experiment.

Its four core modules, import, culture, assay and export, perfectly combined, smoothly completed most of the processes required for the development of monoclonal cell lines. Automatic operation can avoid the interference of human factors in the development process of conventional monoclonal cell lines and improve the stability and repeatability, making the final results more scientific and reliable.

Beacon single cell photoconductive system is the only comprehensive system that can directly and non destructively control single cells, and conduct full-automatic culture, detection, cloning, export and in-depth research. It will greatly improve the research results of researchers, and help the Department to make breakthroughs in the development of monoclonal cell lines, save time, and do more projects.

application area

1 discoveryof antiviral neutralizing antibody

At present, there are three strategies to obtain antiviral neutralizing antibody: 1. Obtain it from the blood of convalescent patients; 2. Immunize mice with virus protein through the antibody discovery process; 3. Obtain it by screening human natural antibody library. The neutralizing antibody obtained by the first strategy has the best effect and the shortest time. In order to obtain antiviral neutralizing antibody quickly and efficiently, single B cells were screened and sequenced. Single B cell antibody discovery has many advantages and become a hot new direction in the field of antibody discovery, but limited by the conventional technology platform, it is difficult to achieve the separation, screening and recovery of single B cell. After a single B cell is introduced into nanopen, the secretion of antigen-specific antibody can be detected in parallel, and the related functional verification can be carried out, and the target B cell can be specifically exported. With the downstream single cell mRNA sequencing, the cell phenotype screening can be completed in two days and the corresponding heavy and light chain sequence of antibody can be obtained. Compared with the traditional antibody preparation technology, beacon platform has the advantages of high cell utilization, high recovery efficiency, support for humanization, heavy chain and light chain sequence information matching.

2 antibody engineering

In the process of antibody drug development, in order to make the antibody have the best structure and activity, it is often necessary to carry out several rounds of transformation and optimization. For example, by means of genetic engineering, antibody genes are processed and transformed, transferred to engineering cells for reassembly, and functional screening and analysis, so as to confirm whether the transformation meets the needs. The traditional screening after transfection takes weeks, while the beacon single cell photoconductive system can be realized in only 3-5 days, which greatly saves the time cost of antibody engineering optimization process.

3 tumor immunotherapy

In the immunotherapy of tumor, in order to verify the efficiency of cell transformation and optimize the dosage of cells for treatment, the industry has been looking for a technical means to effectively screen and verify the transformed T lymphocytes. Beacon single-cell photoconductive system can activate T lymphocytes specifically and detect the secretion of specific cytokines, so as to achieve the functional screening of T lymphocytes. After screening, T lymphocytes can be derived, which can greatly improve the screening efficiency by combining with downstream gene expression profiling, TCR sequencing, TCR engineering and other means.

Gene editing technology is an important research method of immunology, cell biology and other disciplines, and also an important way of species improvement, disease prevention, treatment and other fields. In the process of conventional gene editing experiment, in the process of screening after transfection, it takes 1-2 months for a large number of planking, using imaging or biochemical methods for monoclonal screening and identification, while the beacon single cell photoconductive system only needs 5 Days, we can screen the target clone automatically and on a larger scale, which can improve the efficiency of the experiment, save the human cost, and get more reliable and detailed experimental results.

Study on 5-cell co culture

Cell co culture technology in vitro can simulate the microenvironment generated in vivo, so as to better observe the interaction between cells, cells and culture environment and explore the role of drugs


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Website:www.laidebote.com


Fax:010-61841882


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Living cells