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Company name: Ladd Porter (Beijing) Biotechnology Co., Ltd.

Contact: Zhang Wei

Tel: 010-61841882

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Lightning real time single cell immunology research system

Lightning real time single cell immunology research system

  • Taxonomy:Lightning real time single cell immunology research system

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  • Date of release:2020/02/26
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For a long time, tumor immunotherapy has been committed to how to overcome the mechanism of tumor immune escape and reawaken immune cells to clear cancer cells. Among them, how to help T cells overcome tumor induced immunosuppression and achieve activation is the key to treatment research. The lightning real-time single cell immunology research system developed by Berkeley lights focuses on T cell phenotypes and cells Factor detection workflow provides researchers with a comprehensive set of single cell level T cell research methods.


1. Combined with MHC tetramer technology, target T cells can be labeled at the single cell level in microfluidic environment. Compared with the traditional FACS detection, lightning provides a fast and mild method for antigen-specific selection of T cells.


2. It can directly detect the expression level of single cytotoxic T cell surface markers (CD8 + / CD4 + / CD137 +, etc.) and the real-time secreted cytokines (IFN - γ / TNF α / IL2, etc.).


3. The cytotoxic activity of activated cytotoxic T cells can be evaluated on-line.


4. It provides a simplified and efficient development process of T cell receptor with high affinity and specificity. Only 2 days can be used to complete the online integration of specific T cell sorting (TCR screening) completed in the original 1-3 months Cloning and functional verification tests help researchers find the best T cell antigen receptor that can effectively bind to the target antigen in the shortest time.


5. In the research related to immunogenomics, the system can help researchers selectively export the target T cell / single T cell clone population after analysis and evaluation for subsequent downstream sequencing and other experimental operations.


In general, in the application of T cell modification and car-t / tcr-t cell therapy technology, lightning, with its unique single cell level research, helps researchers to complete the real-time and efficient work flow of activity function analysis, culture and cloning of T cells and their receptors after transformation, greatly accelerating the whole fine cell in the way of digital cytology The progress of cellular immunotherapy.


Application example: Lightning helps the research of car-t, tcr-t and other T cells


Two of the most common methods of targeted treatment of tumor by transgenic T cells are to introduce new T cell receptor (TCR) or chimeric antigen receptor (car) into genetically engineered T cells. Lightning real-time single cell immunology research system helps researchers to study and construct car / TCR vector at the single cell level quickly and efficiently, and accelerates the process of T cell gene transformation. Tcr-t is a traditional immunoadoptive therapy that only increases the number of effector cells. It is to introduce tumor antigen-specific TCR gene into T cells, so as to directly modify the "joint" TCR of T cells combining with tumor antigens, and increase the specificity of effector cells, so that the binding affinity of effector cells and tumor cells is also greatly increased




To improve the technology of targeted anti-tumor therapy, which is finally infused into patients after amplification in vitro. This kind of gene modified T-lymphocytes, which can recognize specific targets by infusion, endows the immune system with new natural immune activity. It can not only kill tumors quickly, but also avoid the delayed effect of traditional vaccines and T-lymphocyte checkpoint treatment methods. However, the efficiency of target TCR gene therapy is relatively low. Researchers have been focusing on finding effective TCR receptor for tumor target antigen clone affinity and optimizing TCR transformation efficiency. In the following figure is the TCR development process. Compared with the traditional development process, a large number of high affinity and specific T cell receptor development and research processes are found based on the lightning system.




The development process of TCR on Lightning greatly simplifies and improves the existing complex and cumbersome methods. Only two days can be used to complete the online integration of specific T cell efficient sorting (TCR) in the original 1-3 months




Screening) and cloning and functional verification tests help researchers to find the best T cell antigen receptors (TCR candidates) that can effectively bind to the target antigens in the shortest time, and finally directly relate to the downstream sequencing process to help researchers directly obtain TCR sequences that specifically recognize tumor antigens for TCR drug development or vaccine research. Of course, not only TCR development, but also lightning's unique single cell level research methods in other T cell modifications and related applications of car-t cell therapy technology help researchers to reconstruct T cells and their receptors




The work flow of activity function analysis, culture, cloning and so on was completed in real time and efficiently, which greatly accelerated the whole process of cell immunotherapy research by means of digital cytology.


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Address: Room 602a, floor 6, building 2, xin2, Dongsanhuan North Road, Chaoyang District, Beijing

Website:www.laidebote.com


Fax:010-61841882


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Living cells