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Company name: Ladd Porter (Beijing) Biotechnology Co., Ltd.

Contact: Zhang Wei

Tel: 010-61841882

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High-throughput Cytological Discovery System Cell Voyager CV8000

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High-throughput Cytological Discovery System Cell Voyager CV8000

  • Taxonomy:Yokogawa

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  • Date of release:2018/08/30
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Detailed introduction

CV8000 (High-throughput Cytological Discovery System Cell Voyager CV8000) High-throughput Cytological Discovery System Cell Voyager CV8000 can get images of cells in microplates and slide glasses and analyze the increase or decrease of the number of target molecules inside the cells, the change of co-localization, cell motility, morphology change and so on from the acquired images. This method conducts the optimization of drug candidates, pathway analysis, toxicology tests, cell-based large scale screening and the quality control of cells. In addition, the CV8000 comes with CellPathfinder, a new program that can analyze images of unlabeled cells and 3D images of samples. With these features, the CV8000 improves the efficiency of drug discovery research and biomedical research on leading-edge subjects such as iPS and ES cells.

Features


1. High-speed and high-resolution 3D cell imaging

  • The sliced images of cells can be acquired with high-speed and high signal to noise ratio by the microlens enhanced Nipkow disk confocal scanning technology. High-speed imaging and analysis of 3D data of cells exceed the conventional high content analysis.

  • CV8000 optionally mountsφ25μm pinhole disk that is suitable for low magnitude confocality as well as standard φ50μm pinhole disk. It can cover the wide range of samples.

  • Users can choose the pinhole disk matching to the purpose of the experiments. Z-resolution in thick samples is improved by using 25μm pinhole disk.

  • It can also mount up to four large field sCMOS cameras. Wide image of multi-labeled sample can be acquired by one shot.

High-throughput Cytological Discovery System Cell Voyager CV8000

Analysis system

2. Live cell imaging function

  • The microlens enhanced Nipkow disk confocal scanning technology excites a fluorescence dye with high-efficiency with very low energy laser as well as high-speed confocal imaging. This feature greatly fits for the live cell imaging.

  • Based on accumulated technology in Yokogawa, the performance of control for temperature, concentration of CO2 and humidity retention has been improved. It can let the users to aquire the cell images under longer time period and better environment.

  • Moreover,with the digital phase contrast technology and machine learning method, label free analysis of cells can be performed.


Time lapse/kinetic assays

  • Stage incubator enables live cell imaging over 3 days without supplying additional water

  • Disposable tip type liquid dispenser for kinetic assays

  • Hypoxia experiments and FRET

Dual rotary laser confocal high definition analysis system

Calcium response

  • Fast kinetic responses can be visualized by applying drugs during time lapse recordings.

Dual rotary laser confocal high definition analysis system

Ionomycin was applied to A10 cells labeled with Fluo-4 during recording with 100ms intervals.

High throughput

  • Wide-view sCMOS camera, up to 4 cameras

  • Approx. 1 minute to acquire 4-color images of 96 wells

Living cells

Living cells

Water immersion objectives

Water immersion objective lens provides high signal to noise ratio and high resolution imaging. Micro structure such as organelle can be observed and analyzed. Also, by effect of refractive matching, image resolution in deep region of 3D sample improved dramatically.

  • 40x and 60x water immersion objectives provide brighter and higher resolution images.

  • Automated water supply

High-throughput Cytological Discovery System Cell Voyager CV8000

3. Image analysis function(High content analysis software “CellPathfinder”)

  • Preset analysis menus for a variety of applications

  • Flexible graph functions to display analysis results

Direct link between chart and object image

High-throughput Cytological Discovery System Cell Voyager CV8000


Machine Learning

  • Software learns the features of the sample objects collected by users.

Analysis system

Analysis system

Label-free analysis

  • DPC (Digital phase contrast) function is a powerful tool to analyze unstained bright field samples.

Dual rotary laser confocal high definition analysis system


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Fax:010-61841882


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Living cells