Juno System

Achieve an Economy of Scale

Increase productivity and efficiency with automated, cost-effective, and easy-to-use workflows for targeted DNA next-generation sequencing (NGS) library preparation, gene expression analysis and genotyping by allele-specific PCR.

Using the Juno™ Targeted DNA Sequencing Library Preparation System, including Targeted DNA Seq Library Preparation reagents, you can produce dozens to hundreds of sample libraries daily, with each sample enriched for up to 4,800 specific amplicons covering user-defined genes or genomic loci. Optimized for use with Illumina® sequencing systems in combination with Fluidigm-supplied sample barcodes, Juno enables accurate sequencing of more samples—faster and more affordably than ever before.

Juno also serves as a universal controller to prepare integrated fluidic circuits (IFCs) for both gene expression and genotyping analysis when used together with the Fluidigm Biomark™ HD system. While providing significant advantages in sample throughput for the analysis of dozens to hundreds of DNA variants or RNA transcripts per sample, the Juno system also significantly reduces reaction volumes and required hands-on time, resulting in substantial savings over traditional methods.

Juno System characteristics:

(1) Scale:
Generate dozens to hundreds of NGS-ready libraries in a single run using Juno and either the 48 sample or 192 sample Library Prep IFC.

(2) Save:
Boost affordability by reducing reagent and sample volumes and labor requirements, for lower overall cost.

(3) Simplify:
Easily design assay panels for targeted NGS, SNP genotyping or gene expression to detect DNA variants, mRNAs or miRNAs—from a few targets to several thousand.

Technology:

Juno integrates IFC control and thermal cycling to enable scalable, automated NGS library preparation for Illumina sequencers and IFC preparation for both gene expression and genotyping analysis using the Fluidigm Biomark HD.