Company name: Ladd Porter (Beijing) Biotechnology Co., Ltd.
Contact: Zhang Wei
Mobile phone: 18101288748
Address: room 2071, 2 floor, 5 building, No. 5 hospital, Beijing, Chaoyang District.
The Revolution at Hand
Individual cells vary as much as the researchers who study them. Traditional methods of extraction, isolation by pipette, and processing cells are laborious, time-consuming and prone to introducing errors. In addition, averaging cell pools masks many important functions within a single cell. Geneticists and clinical researchers have been looking for a complete workflow that can examine and differentiate individual cells and group them according to unique genomes and transcriptomes while minimizing technical noise.
The C1™ system takes an entirely new approach, based on innovative Fluidigm® microfluidic technology that enables you to rapidly and reliably isolate, process, and profile individual cells for genomic analysis. For the first time, you can extract, reverse transcribe, preamplify, and ultimately detect and analyze cell activity using just one technology, reducing the variability caused by multiplatform technical errors.
The C1 enables you to study cell differentiation, measuring individual cell responses to specific stimuli, verify critical disease biomarkers, validate RNAi knockdown, and conduct candidate drug screens.
Fig 1. The C1 IFC uses integrated thermal and pneumatic control at
nanoliter scale to perform every step of the C1 workflow. From
single-cell capture to amplification, the C1 IFC operates without
intervention. No reagent mixing, transfer or addition is required. It is
easy to load, requires minimal hands-on time, reduces the risk of
carryover contamination, and provides highly reliable data quality.
C1 System characteristics:
(1) Single-cell precision—greater accuracy to measure differences in gene expression profiles;
(2) Easy to use—simplified cell isolation and preparation with a streamlined workflow and intuitive interface;
(3) Fast—cell input-to-data in less than a day;
(4) All in one—comprehensive, automated workflow generates reproducible and reliable results;
(5) Flexible—expandable into whole transcriptome and variant discovery
Up to 96 Single Cells in Just One Hour:
The C1 system provides a simple plug-and-play workflow to reproducibly prepare 96 individual cells in about one hour of hands-on time.
The C1 system coupled with the Biomark HD system streamlines gene expression analysis through seamless effort and significant time savings with support for up to 96 individual cells across 96 transcripts. With automation and minimal costs, the C1 dramatically increases productivity to further accelerate expression profiling studies. With an optimized protocol, preformulated reagent kit and disposable arrays, you can achieve single-cell accuracy with load-and-go productivity.
The simple user interface and comprehensive C1 workflow will help you:
Step one: CAPTURE CELLS
Load batches of cells in a single pipetting step and rapidly separate into 96 individual chambers for preparation;
Step two: VERIFY
Use an “in-process” quality control checkpoint to verify the number of captured cells and distinguish live and dead cells to preserve data integrity;
Step three: LYSE
Use a rapid direct cell lysis method to save time and cost without RNA purification;
Step four: REVERSE TRANSCRIBE AND PREAMPLIFY
cDNA synthesis and specific target amplification occur on the same sample without reagent mixing and sample transfer;
Step five: HARVEST
All preamplified products are pooled, harvested, and transferred to the Biomark™ HD system for quantitative PCR analysis.
Ladd Bert (Beijing) biology
Address: Ladd Porter (Beijing) Biotechnology Co., Ltd.
Copyright: Ladd Porter (Beijing) Biotechnology Co., Ltd.