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The Avatar System

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The Avatar System

  • Taxonomy:XCell Biosciences

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  • Date of release:2018/08/30
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Detailed introduction

Developing solutions that replicate physiologically relevant conditions The Avatar System Your biological samples are precious and unique. They also require real-life conditions to thrive and generate meaningful results. The Avatar System is the first commercially available cell culture system capable of reproducing the diverse range of in vivo microenvironments found within the human body. By reproducing physiological conditions, the Avatar System cultures cell samples in the most suitable environment to deliver biologically relevant results. Avatar System regulates O2, CO2, pressure, and temperature to recreate the native in vivo conditions in which the sample was derived. These revolutionary capabilities enable the culturing of difficult to grow patient samples, from primary cells, stem cells and tumor cells.

(1) Do you want to rebuild the physiological microenvironment of cells in vitro?
(2) Are you suffering from the low survival rate of primary cells?
(3) Are you bothered by the fact that the number of CTCs is too small to perform more downstream applications?
(4) Do you want to reduce the difference between cytology experiment results and clinical trial results?
(5) Are you eager to improve the efficiency of gene editing, CAR-T, reprogramming of stem cells?

The Avatar System can resolve these problems.

The Avatar System is a revolutionary system for cell-based assays that overcomes limitations of traditional cell culture and replicates the in vivo microenvironment to deliver reproducible, physiologically relevant results.

XCell also offers complete protocols and reagent kits for primary cell culture applications. Xcell's cell-binding substrates are specifically formulated for maintaining and expanding primary cells in culture. Xcell's culture media are chemically defined to closely replicate physiological conditions for maintaining and propagating primary cells.

Individual cell precise control system

Applications:

(1) Primary Cell Research

In standard cell culture process, primary cells often fail to propagate under conditions provided by a standard incubator. These cells exhibit non-native morphology and significantly altered gene expression profiles.

Unlike traditional incubators, the Avatar bioreactor enables users to define physiologically relevant culturing conditions. For pancreatic cancer cells, 2% O2 and 1 PSI culturing conditions resulted in increased tumor colony formation. The average expression of MYCN is significantly increased in pancreatic tumor cells when hypoxia and pressure are applied to culturing conditions. Primary pancreatic tumor cells cultured in Avatar exhibit increased colony number and growth.

The Avatar System

Fig.1 Primary pancreatic tumor cells cultured under hypoxic and pressurized conditions exhibit increased colony number and growth.

(2) Stem Cell Research

In standard incubator, human stem cells can uncontrollably and spontaneously differentiate, leading to changes in cell morphology and decreased expression of the stemness marker NANOG.

The Avatar system can be used to optimize stem cell maintenance and tune for stem cell fate. Human pluripotent stem cell colonies were cultured using the Avatar system to demonstrate how oxygen and hydrostatic pressure can control stem cell fate in a minimally supportive cell culture media. Stem cells cultured at 1.0% O2 and 2.0 PSI maintained their stemness, retaining their cell morphology and express higher level of NANOG. By controlling oxygen and pressure levels during cell culture, human stem cells can be maintained in a stem-like state with nuclear Sox2 and compact colony morphology, even in minimally supportive culture media.

Individual cells

Fig. 2 By controlling oxygen and pressure levels during cell culture, human stem cells can be maintained in a stem-like state with nuclear Sox2 and compact colony morphology, even in minimally supportive culture media.

The Avatar System

Fig. 3 Comparison of stem cell in Avatar system vs. standard incubator.

(3) Compound Screening

Compound screening performed under culturing conditions mimicking the metastatic tumor microenvironment may lead to drug candidates relevant for late-stage cancer patients. Compound screening performed on cell lines using standard culturing conditions may not accurately reflect in-vivo efficacy of the drug candidate. Drug screening assays performed under physiologically-relevant culturing conditions of Avatar often lead to conflicting results when compared to screening assays cultured in traditional incubators.

 Living cells

Fig 4. Drug screening assays performed under physiologically-relevant culturing conditions often lead to conflicting results when compared to screening assays cultured in traditional incubators. (Left: standard incubator; Right: Avatar system)

(4) Circulating Tumor Cells Research

Viable CTCs cultured with standard incubators failed to propagate. Implementation of Xcell's proprietary protocols and reagents resulted in viable and propagating CTC colonies after just 2 weeks when cultured in the Avatar bioreactor. Propagating prostate CTCs cultured under 2.0% O2 and 2.0 PSI show increased expression of AMACR1, a metastasis marker. Viable prostate CTCs enriched using Xcell's Liquid Biopsy Protocol result in proliferating CTC colonies that can be maintained in culture for several months.

Individual cell precise control system


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Address: Ladd Porter (Beijing) Biotechnology Co., Ltd.


Website:www.laidebote.com


Fax:010-61841882


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Living cells