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Company name: Ladd Porter (Beijing) Biotechnology Co., Ltd.

Contact: Zhang Wei

Tel: 010-61841882

Mobile phone: 18101288748


Fax: 010-61841882


Address: Room 602a, floor 6, building 2, xin2, Dongsanhuan North Road, Chaoyang District, Beijing

Green reagent for apoptosis

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Green reagent for apoptosis

  • Taxonomy:Essen BioScience

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  • Date of release:2018/08/30
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Detailed introduction

IncuCyte® Caspase-3/7 Green Apoptosis Assay ReagentOne vial (20 ul) of 5 mM mix and read reagent in DMSO sufficient for 100-200 live-call apoptosis tests in 96-well format

The IncuCyte® Caspase-3/7 apoptosis assay reagent couples the activated caspase-3/7 recognition motif (DEVD) to NucView™488, a DNA intercalating dye to enable quantification of apoptosis over time. The Caspase-3/7 assay reagent is specially formulated for use in the IncuCyte® live-cell analysis system and can be added directly to tissue culture wells using a no wash, mix and read protocol to acquire live cell images of cells undergoing caspase-3/7 mediated apoptosis. In addition, the Caspase-3/7 assay reagent is non-perturbing to cell growth and morphology. When added to tissue culture medium, this inert, non- fluorescent substrate crosses the cell membrane where it is cleaved by activated caspase-3/7 resulting in the release of the DNA dye and green fluorescent staining of nuclear DNA. Kinetic activation of caspase-3/7 can be monitored morphologically using live cell imaging, and quantified using the IncuCyte® basic analyzer.

Green Apoptosis Assay Reagent

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Key word:GreenApoptosisAssayReagent,IncuCyteCaspase,Livingcells

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Living cells