Company name: Ladd Porter (Beijing) Biotechnology Co., Ltd.
Contact: Zhang Wei
Mobile phone: 18101288748
Address: room 2071, 2 floor, 5 building, No. 5 hospital, Beijing, Chaoyang District.
IncuCyte® NucLight Rapid Red Reagent for live-cell nuclear labelingThe IncuCyte Rapid Red Reagent for cell labeling is a cell permeable DNA stain that specifically stains nuclei in live cells and is ideally suited to the mix-and-read, real-time quantification of cell counting.
The IncuCyte Rapid Red Reagent for cell labeling is a cell permeable DNA
stain that specifically stains nuclei in live cells and is ideally
suited to the mix-and-read, real-time quantification of cell counting.
Addition of the IncuCyte NucLight Rapid Red Reagent to normal healthy
cells is non-perturbing to cell growth and morphology and provides
homogenous staining of nuclei. When added to tissue culture medium, the
inert stain crosses the cell membrane and has excellent specificity for
DNA without the need for a wash step. With the IncuCyte® integrated
analysis software, fluorescent objects can be quantified and background
fluorescence minimized. This reagent can be multiplexed with IncuCyte®
Annexin V, Caspase-3/7 or Cytotox reagents for simultaneous readouts of
apoptosis or cytotoxicity alongside cell proliferation.
Simple to use, mix-and-read reagent
Rapid, high efficiency cell labeling
Non-perturbing to cells at optimized dye concentrations
Ideal for homogenous labeling of multiple cell types
The IncuCyte NucLight Rapid Red Reagent has been validated for use with
the IncuCyte® live-cell analysis system, using a simple mix-and-read
protocol, enabling the real-time quantification of cell proliferation.
At optimized concentrations, the reagent is non-perturbing and allows
for long-term fluorescent measurements of cell nuclei and can be
combined with our range of IncuCyte® Cell Health Reagents to quantify
apoptosis or cytotoxicity alongside cell proliferation in a single well.
IncuCyte NucLight Rapid Red Reagent Quick Guide
NucLight Rapid Red Reagent is non-perturbing and enables high-efficiency labeling of cell nuclei
Optimized dye concentrations do not perturb cell health. NucLight Rapid Red Reagent labeled HT-1080 (adherent; left graph) and Jurkat (non-adherent; right graph) cells show equivalent cell growth profiles to non-labelled cells.
Rapid, high efficiency labelling of adherent and non-adherent cell types.
After addition of NucLight Rapid Red Reagent, all cells in the
population are labeled immediately and homogenously. Note the
homogeneous labeling of cell nuclei and the healthy cell morphology.
HT-1080 cells (left image) and Jurkats (right image) show representative
nuclear labeling and red fluorescence.
Multiplex with cell health reagents
Analysis of target tumor cell viability over 48 hours multiplexed with an apoptosis readout.
HT-1080 cells were labeled with IncuCyte NucLight Rapid Red Reagent
and cultured in the presence of IncuCyte® Caspase-3/7 Green reagent.
Cell death (apoptosis) is monitored in real time by measuring the loss
of red object area (left) and increase of green object count
(Caspase-3/7 signal) over time.
Visualize and quantify immune cell clustering and proliferation
Visualize changes in the phenotype of labeled immune cells.IncuCyte
NucLight Rapid Red labeled human PBMC’s activated with anti-CD3
antibody and IL-2 induces T-cell aggregation or “clustering”. Images
shown at are at 0, 60, and 96hrs. User friendly IncuCyte® software
enables accurate quantification of cell clustering over time.
Ladd Bert (Beijing) biology
Address: Ladd Porter (Beijing) Biotechnology Co., Ltd.
Copyright: Ladd Porter (Beijing) Biotechnology Co., Ltd.