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Company name: Ladd Porter (Beijing) Biotechnology Co., Ltd.

Contact: Zhang Wei

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IncuCyte ClearView 96-well Chemotaxis Plate

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IncuCyte ClearView 96-well Chemotaxis Plate

  • Taxonomy:Essen BioScience

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  • Date of release:2018/08/30
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Detailed introduction

Each well of a ClearView plate provides an optically clear surface for label-free imaging and analysis of chemotactic cell migration using the IncuCyte® Live-Cell analysis system.

Overview of the ClearView Chemotaxis Plate design

The IncuCyte® ClearView Chemotaxis Plate combines the throughput of transmembrane assays with the optical clarity of microfluidics devices. Each well of a ClearView plate provides an optically clear surface for label-free imaging and analysis of chemotactic cell migration using the IncuCyte® live-cell analysis system.

The ClearView membrane contains 96 laser etched pores with a diameter of 8µm. Cells are added to the upper chamber and chemoattractant to the lower reservoir plate. Chemotactic transmembrane migration is visually monitored within your incubator and automatically analyzed using the IncuCyte live-cell analysis system.

Chemotaxis Plate

Plate composition

Each IncuCyte® ClearView Cell Migration Plate is composed of :

One plate lid (A)
One 96-well ClearView membrane insert (B)
One 96-well reservoir plate (C)

Key advantages

Visualize every cell

The specially designed ClearView membrane enables you to monitor every cell throughout your experiment in real time – gain deep phenotypic insight into cell movement and morphology.

IncuCyte ClearView

Monitor every cell in your experiment. The ClearView membrane enables label-free visualization and quantitation of chemotaxis using IncuCyte® system. Each membrane contains 96 precision engineered holes in a regular grid array. Three pores are highlighted in yellow.

Low cell usage ideal for rare, expensive and primary cell populations

Use 10- to 100-fold fewer cells than standard transmembrane (e.g. Boyden Chamber) approaches

Imaging system

Lower cell usage. Cell usage comparison for the ClearView Plate and Corning® Transwell® system. Jurkat T cell chemotaxis toward SDFα-1 was measured in both systems (2,000 to 20,000 cells/well); however, no signal was observed with the Transwell® plate. A minimum of 150,000 cells/well was required to observe chemotactic migration using the Corning® Transwell® system.

Biologically relevant surface-mediated migration

Cells must migrate across a biologically relevant surface towards the chemoattractant due to the low pore density of the specially designed ClearView membrane (pore density <2% that of Boyden chamber transwells)

The ClearView plate has been validated with a wide range of biomatrix coatings, cell types and chemoattractants

Chemotaxis Plate

Cells must interact with substrate to reach a pore. Time-lapse images of a Jurkat T cell (outlined in white) migrating across a Matrigel® coating towards a pore leading to the chemoattractant SDF-1α (orange circle). White arrows indicate the direction of Jurkat movement in each frame. In the final image the Jurkat T cell has entered the pore and moved to the lower chamber.

Stable and sustained gradients  hours     Living cells
Chemotactic gradients are stable and maintained over 72

Chemotaxis Plate

Long-term stable gradients compared to traditional Boyden chamber transwells. (A) Gradient stability was monitored over 72 hours using an Alexa Fluor® 594 dextran (10 kDa). After 4 hours a rapid loss in gradient was measured in the Corning® Transwell® system. In contrast, >80% of the gradient was maintained in the IncuCyte® ClearView plate at 72 hours. (B) FBS gradients pre-establised in a ClearView plate for 24, 48, and 72 hours induced equivalent rates of directional migration in HT1080 cells indicating long-term and stable gradients across the ClearView membrane. Rates were slightly faster than for cells migrating towards gradients that had not been pre-established. This is likely a result of increased cell health when serum starved cells are plated in established gradients.

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Key word:IncuCyteClearView,ChemotaxisPlate,Imagingsystem

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Living cells