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How to detect living cells

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How to detect living cells

Date of release:2020-08-04 Author: Click:

Living cells are the cells we need to metabolize, and they have many effects on us, and they are also one of the necessary cells in our bodies, so how should living cells be detected? let Redbert show us next.

Beijing living cell culture equipment

1. Living dye-methylene blue solution. 

In previous textbooks, living dye methylene blue solution can be used to identify all metabolic cells. Using the principle of exchange adsorption, only living cells can complete exchange adsorption. 

2. Staining of embryos-red ink. 

The cell membrane is selectively permeable when the cell is alive, so the organic dye is generally not absorbed, so it can be used to identify the life or death of the seed, such as the corn cell is alive, the embryo is not stained red, and the endosperm is stained red. 

3. Microscopic observation-plasmalemma separation and restoration. 

Living and mature plant cells have obvious plasmolysis and recovery. 

4. Microscopic observation-cytoplasmic circulation. 

Through the observation of cytoplasmic flow experiment, we can know that only living cells have the phenomenon of cytoplasmic circulation.

Study on living immune cells

5. Dye-Trypan Blue. 

In the textbook of the people's Education Edition, trypan blue stain, also known as cone blue, is an anionic dye that cannot penetrate the complete cell membrane, so after staining with trypan blue, dead cells can only be stained, but living cells will not be stained. 

6. Yeast cells-methylene blue dye. 

The difference in metabolism between dead and living cells is the basis for the identification of yeast cells with methylene blue dye. 

Methylene blue is a non-toxic dye with blue oxidation and colorless reduction. 

Due to the role of metabolism in living cells, there is a strong reducing ability in the cells, which can change methylene blue from blue oxidation to colorless reduction, so the living yeast cells stained with methylene blue are colorless, while dead cells or old cells with slow metabolism, because of their lack of reductive ability or extremely weak reductive ability, methylene blue is in the oxidizing state, so it is dyed blue or light blue. 

7. Fluorescence staining method. 

Fluorescein diacetate (FDA) is a commonly used dye for identifying the viability of cultured animal and plant cells and plant cell protoplasts, and its staining mechanism also takes advantage of the metabolic differences between dead and living cells. 

FDA itself does not produce fluorescence, does not have polarity, and can freely infiltrate into and out of the intact cell membrane. 

When FDA enters a living cell, it is decomposed by lipase in the cell to produce a polar substance that can produce fluorescence-fluorescein, which can not freely penetrate the living cell membrane and accumulate in the cell membrane, which makes the active cells produce green fluorescence, while the inactive cells can not produce fluorescence because they can not decompose FDA.

Which is a good place to study living cells in Beijing?

If we want to cultivate living cells, we can only grow active cells at a certain density. For cells separated from cells, we need to do some tests first. Click on the dynamic imaging of living cells for details.

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Key word:DetectionoflivingcellsinBeijing,StudyonlivingcellsinBeijing,WhichisagoodplacetostudylivingcellsinBeijing?

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Address: Room 602a, floor 6, building 2, xin2, Dongsanhuan North Road, Chaoyang District, Beijing

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Living cells